Mutations in PCR products are detected by the presence of heteroduplexes optimally detected by our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile compared to a reference sample. Hi-Res Melting® (HRM) on the LightScanner® is also being used for mutation discovery in tumor samples, yielding information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.

Use the links below to learn how your research can get there faster with our complete line of products.

Instrument

LightScanner Instrument
LightScanner® System

  • Hi-Res Melting Instrument
  • 96- or 384-well plate system
  • Results in less than 5 minutes
  • Software automatically analyzes each sample and defines the standards and variants
Chemistry

LightScanner Master Mix

LightScanner Master Mix

  • A pre-optimized solution specifically designed to deliver superior PCR performance for Hi-Res Melting applications on the LightScanner Instrument.

LCGreen + Melting Dye

LCGreen® Plus Melting Dye

  • LCGreen dye is specifically designed for Hi-Res Melting curve analysis to detect DNA sequence variants (mutations, polymorphisms, etc).